Kurzfassung

Tissue culture systems are tools of choice to study cell or tissue behaviour under physiological and/or pathological conditions and to perform pharmacological tests. We are working on a transgenic mouse (Hu-Bcl2 transgenic mouse, line 71) whose retina undergoes early postnatal degeneration linked to the prior death of Müller glial cells. In order to know if the retinal degeneration occurs similarly in culture as in the animal, we generate protein patterns to find protein or peptide biomarkers...Tissue culture systems are tools of choice to study cell or tissue behaviour under physiological and/or pathological conditions and to perform pharmacological tests. We are working on a transgenic mouse (Hu-Bcl2 transgenic mouse, line 71) whose retina undergoes early postnatal degeneration linked to the prior death of Müller glial cells. In order to know if the retinal degeneration occurs similarly in culture as in the animal, we generate protein patterns to find protein or peptide biomarkers which could characterize this degeneration in fresh tissue to and compare it to the patterns obtained with tissue maintained in culture.
Protein profiles will be performed by surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) ProteinChip Array technology. Biomarker pattern will be detected and compared that characterize retinal degeneration of Hu-Bcl2 transgenic mice as a function of time in fresh and cultured tissue conditions. » weiterlesen» einklappen