Kurzfassung

The process of microvascularization represents a potential target for therapeutic intervention of a number of diseases, including diabetes mellitus, which has not yet been sufficiently explored. Preliminary results revealed that high glucose decreases the ATP level of endothelial cells. In this project, a first approach to mitigate the impaired function of endothelial cells at high glucose based on the application of polyphosphate which acts as a “metabolic fuel” in the extracellular space....The process of microvascularization represents a potential target for therapeutic intervention of a number of diseases, including diabetes mellitus, which has not yet been sufficiently explored. Preliminary results revealed that high glucose decreases the ATP level of endothelial cells. In this project, a first approach to mitigate the impaired function of endothelial cells at high glucose based on the application of polyphosphate which acts as a “metabolic fuel” in the extracellular space. The effects of glucose and polyphosphate on tube formation of endothelial cells (HUVEC) seeded onto a solidified basement membrane extract matrix, cell viability/survival and the expression of the genes encoding for matrix metalloproteinases will be elucidated. It is expected that the results of this project will provide a novel, hitherto unexpected solution for the prevention of the adverse effects of high glucose on endothelial cell function at the level of microvascularization in diabetes mellitus.
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