Human CD4 CD25 regulatory T cells : proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function
Blood. Bd. 110. H. 5. Stanford, Calif.: HighWire Press 2007 S. 1550 - 1558
Erscheinungsjahr: 2007
ISBN/ISSN: 0006-4971
Publikationstyp: Zeitschriftenaufsatz
Sprache: Englisch
Doi/URN: 10.1182/blood-2007-01-069229
| Geprüft: | Bibliothek |
Inhaltszusammenfassung
CD4( )CD25( )Foxp3( ) regulatory T cells (CD25( ) Treg cells) direct the maintenance of immunological self-tolerance by active suppression of autoaggressive T-cell populations. However, the molecules mediating the anergic state and regulatory function of CD25( ) Treg cells are still elusive. Using differential proteomics, we identified galectin-10, a member of the lectin family, as constitutively expressed in human CD25( ) Treg cells, while they are nearly absent in resting and activated CD4(...CD4( )CD25( )Foxp3( ) regulatory T cells (CD25( ) Treg cells) direct the maintenance of immunological self-tolerance by active suppression of autoaggressive T-cell populations. However, the molecules mediating the anergic state and regulatory function of CD25( ) Treg cells are still elusive. Using differential proteomics, we identified galectin-10, a member of the lectin family, as constitutively expressed in human CD25( ) Treg cells, while they are nearly absent in resting and activated CD4( ) T cells. These data were confirmed on the mRNA and protein levels. Single-cell staining and flow cytometry showed a strictly intracellular expression of galectin-10 in CD25( ) Treg cells. Specific inhibition of galectin-10 restored the proliferative capacity of CD25( ) Treg cells and abrogated their suppressive function. Notably, first identified here as expressed in human T lymphocytes, galectin-10 is essential for the functional properties of CD25( ) Treg cells.» weiterlesen» einklappen
Autoren
Klassifikation
DFG Fachgebiet:
2.21 - Mikrobiologie, Virologie und Immunologie
DDC Sachgruppe:
Medizin
