Insights into aphid prey consumption by ladybirds: Optimising field sampling methods and primer design for high throughput sequencing
PLOS ONE. Bd. 15. H. 7. 2020
Erscheinungsjahr: 2020
ISBN/ISSN: 1932-6203
Publikationstyp: Zeitschriftenaufsatz
Doi/URN: 10.1371/journal.pone.0235054
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Inhaltszusammenfassung
Elucidating the diets of insect predators is important in basic and applied ecology, such as for improving the effectiveness of conservation biological control measures to promote natural enemies of crop pests. Here, we investigated the aphid diet of two common aphid predators in Central European agroecosystems, the nativeCoccinella septempunctata(Linnaeus) and the invasiveHarmonia axyridis(Pallas; Coleoptera: Coccinellidae) by means of high throughput sequencing (HTS). For acquiring insights...Elucidating the diets of insect predators is important in basic and applied ecology, such as for improving the effectiveness of conservation biological control measures to promote natural enemies of crop pests. Here, we investigated the aphid diet of two common aphid predators in Central European agroecosystems, the nativeCoccinella septempunctata(Linnaeus) and the invasiveHarmonia axyridis(Pallas; Coleoptera: Coccinellidae) by means of high throughput sequencing (HTS). For acquiring insights into diets of mobile flying insects at landscape scale minimizing trapping bias is important, which imposes methodological challenges for HTS. We therefore assessed the suitability of three field sampling methods (sticky traps, pan traps and hand-collection) as well as new aphid primers for identifying aphid prey consumption by coccinellids through HTS. The new aphid primers facilitate identification to species level in 75% of the European aphid genera investigated. Aphid primer specificity was highin silicoandin vitrobut low in environmental samples with the methods used, although this could be improved in future studies. For insect trapping we conclude that sticky traps are a suitable method in terms of minimizing sampling bias, contamination risk and trapping success, but compromise on DNA-recovery rate. The aphid diets of both field-captured ladybird species were dominated byMicrolophium carnosum, the common nettle aphid. Another common prey wasSitobion avenae(cereal aphid), which got more often detected inC.septempunctatacompared toH.axyridis. Around one third of the recovered aphid taxa were common crop pests. We conclude that sampling methodologies need constant revision but that our improved aphid primers offer currently one of the best solutions for broad screenings of coccinellid predation on aphids. » weiterlesen» einklappen
