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Establishment of group-specific PCR primers for the identification of freshwater macroinvertebrates

CONSERVATION GENETICS RESOURCES. Bd. 5. H. 4. 2013 S. 1091 - 1093

Erscheinungsjahr: 2013

ISBN/ISSN: 1877-7252

Publikationstyp: Zeitschriftenaufsatz

Doi/URN: 10.1007/s12686-013-9998-1

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Inhaltszusammenfassung


Analysis of the diversity of the species communities and food webs is essential for a better understanding of the ecosystem and PCR based methods are often used for taxonomic identifications. Here, we describe the development of 21 group-specific primers to amplify short sections of genes encoding nuclear ribosomal DNA from several freshwater invertebrate taxa as a potential approach to identify the prey of invertebrate predators, specified on 130 taxa of freshwater macroinvertebrates. Our pr...Analysis of the diversity of the species communities and food webs is essential for a better understanding of the ecosystem and PCR based methods are often used for taxonomic identifications. Here, we describe the development of 21 group-specific primers to amplify short sections of genes encoding nuclear ribosomal DNA from several freshwater invertebrate taxa as a potential approach to identify the prey of invertebrate predators, specified on 130 taxa of freshwater macroinvertebrates. Our primers cover a broad range of aquatic invertebrate taxa and may provide a useful tool for detecting target groups in mixed-DNA samples, making them also suitable for prey species identification in dietary samples. » weiterlesen» einklappen

Autoren


Classen, Silke (Autor)

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