Starten Sie Ihre Suche...


Wir weisen darauf hin, dass wir technisch notwendige Cookies verwenden. Weitere Informationen

Membrane labeling by colored lipid-analogs and photoaffinity-labeling of membrane-proteins by colored hydrophobic azido-probes

Fresenius' journal of analytical chemistry. Bd. 343. H. 1. Berlin: Springer 1992 S. 173

Erscheinungsjahr: 1992

ISBN/ISSN: 0937-0633

Publikationstyp: Zeitschriftenaufsatz

Sprache: Englisch

Doi/URN: 10.1007/BF00332104

Volltext über DOI/URN

Geprüft:Bibliothek

Inhaltszusammenfassung


Membrane and membrane protein markers were synthetized from phospholipids and lipid analogs. The labels contained a absorbant or fluorescent group for visualization of the membranes or labeld mebrane protein within, e.g. a Dansyl or Dabsyl group. The doubled labeling compounds for protein photo-labeling contained an azido-residue. Thus the hydrophobic dye, e.g. 1-Dansyl-8-azido-naphtalene, can be attacched at any time to the protein within microseconds by flash photolysis, e.g. in different p...Membrane and membrane protein markers were synthetized from phospholipids and lipid analogs. The labels contained a absorbant or fluorescent group for visualization of the membranes or labeld mebrane protein within, e.g. a Dansyl or Dabsyl group. The doubled labeling compounds for protein photo-labeling contained an azido-residue. Thus the hydrophobic dye, e.g. 1-Dansyl-8-azido-naphtalene, can be attacched at any time to the protein within microseconds by flash photolysis, e.g. in different physiological states of the membrane.» weiterlesen» einklappen

Autoren


Nawroth, Thomas (Autor)

Klassifikation


DDC Sachgruppe:
Biowissenschaften, Biologie